Mouse B lymphocyte activation of proliferation and cyclic nucleotides at early stages of activation
نویسندگان
چکیده
منابع مشابه
Activation of mouse B lymphocyte by proteins containing hexahistidine.
Many recombinant proteins are produced as a fusion protein tagging hexahistidine, which has been used for studying their biological function both in vitro and in vivo. Unexpectedly, we observed activation of BALB/c mouse splenocytes when treated with hexahistidine-tagged recombinant proteins. This activation was hexahistidine-specific since the anti-pentahistidine antibody completely neutralize...
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Background: MicroRNA-155 (miR-155) is upregulated during T cell activation, but the exact mechanisms by which it influences CD4+ T cell activation remain unclear. Objective: To examine whether the B and T lymphocyte attenuator (BTLA) is a target of miR-155 during naïve CD4+ T cell activation. Methods: Firefly luciferase reporter plasmids pEZX-MT01-wild-type-BTLA and pEZX-MT01-mutant-BTLA were ...
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Bone marrow cells were examined by double immunofluorescent labeling techniques to detect determinants for the B lineage monoclonal antibody, 14.8, the nuclear enzyme, terminal deoxynucleotidyl transferase (TdT), cytoplasmic mu chains (c mu), and surface mu (s mu). In 8-9-wk-old C3H/HeJ mice, 14.8+ cells totalled 22.2% of all marrow cells (35 X 10(5) cells/femur). While many 14.8+ cells were c ...
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Background: The parthenogenetic of oocytes is important in cloning research, as artificial activation of oocytes is an essential component of nucleus transfer protocols. Hydrostatic pressure can act as a mechanical stimulator that rearranges egg contents. Ethanol promotes a single intracellular Ca2+ increase of greater and longer amplitude than the initial increase observed at fertilization. In...
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ژورنال
عنوان ژورنال: Biopolymers and Cell
سال: 1988
ISSN: 0233-7657,1993-6842
DOI: 10.7124/bc.00023d